The former London mayor said the United Kingdom buys so many products from Europe that the countries that sell them would never erect trade barriers.
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The Leave campaign has put forward the same line-up as a previous debate: Boris Johnson, Labour MP Gisela Stewart and the energy minister Andrea Leadsom.
Some of the most heated clashes came over immigration – seen as a strong area for the Leave camp.
“Your campaign hasn’t been Project Fear”.
Mr Johnson hit back – pointing out the current London Mayor had previously said that those concerned about immigration should not be accused of prejudice.
“When you’ve got numbers running at 184,000 net from the European Union, 77,000 coming without even the offer of a job a year ago, it’s obviously time to take back control”.
He said the Leave campaign had failed to outline a plan for Britain’s economy and trade after Britain left the EU.
“That’s scaremongering, Boris, and you should be ashamed … you are using the ruse of Turkey to scare people to vote Leave”, Mr Khan added.
Each politician will make an opening and closing statement, before taking questions from the audience.
She said that more European Union nations would face economic collapse, because of the failure of the Euro single currency, with wealthier nations such as the United Kingdom forced to pay to help them.
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“Even those who want us to leave admit that it’s a big gamble and if there’s one thing you should remember when you go to vote it’s this – if we as a country decide to quit then we are out for good, there’s no going back”.
