The Czech has been sidelined since June after suffering a knee problem while on global duty and was expected to return to full training later in August.
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However, Rosicky’s agent confirmed to website fotbal.idnes.cz the player had now had “arthroscopic knee surgery”, but it is understood any projected recovery time is yet to be determined.
The surgery took place last week.
However, Rosicky could now be unavailable for a further four weeks following the procedure.
“He’s had some treatment, though not anything big, but time is against us”, said Miroslav Pelt, chairman of the Football Association of the Czech Republic.
The Gunners are already without Jack Wilshere after the England worldwide once again damaged his ankle, and they now face the prospect of losing another central midfielder early on in the season.
Arsenal manager Arsene Wenger had said in July that Rosicky picked up “a minor injury” while playing for his country against Iceland.
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The defender missed last Sunday’s 2-0 defeat at home to West Ham with a slight thigh injury.
